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c2 h600l confocal microscope  (Nikon)


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    Structured Review

    Nikon c2 h600l confocal microscope
    C2 H600l Confocal Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 59743 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/h600l+confocal+microscope/Objectives/pmc11021486-252-10-9
    Average 99 stars, based on 59743 article reviews
    c2 h600l confocal microscope - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Incubation:

    Article Title: Ca 2+ binding to F‐ATP synthase β subunit triggers the mitochondrial permeability transition
    Article Snippet: Embryos were pre‐incubated with equilibration buffer (TUNEL kit, Promega) for 10 min and then, incubated for 1 h in the dark at 37°C in equilibration buffer supplemented with fluorescein‐12‐dUTP mix and rTdT enzyme (TUNEL kit, Promega). .. The reaction was stopped by incubation in a solution containing 0.3 M NaCl, 0.03 M trisodium citrate, pH 7, for 20 min, then embryos were transferred onto glass slides, set in 0.8% low melting agarose, laterally oriented, and analyzed using a NIKON H600L confocal microscope equipped with Laser 488 (excitation 488 nm and emission 520 nm) to visualize GFP and a 40× objective. .. Apoptotic nuclei in entire embryos (head, trunk, yolk and tail) were counted using VOLOCITY 6.0 software (PerkinElmer, Waltham, MA, USA).

    Microscopy:

    Article Title: Ca 2+ binding to F‐ATP synthase β subunit triggers the mitochondrial permeability transition
    Article Snippet: Embryos were pre‐incubated with equilibration buffer (TUNEL kit, Promega) for 10 min and then, incubated for 1 h in the dark at 37°C in equilibration buffer supplemented with fluorescein‐12‐dUTP mix and rTdT enzyme (TUNEL kit, Promega). .. The reaction was stopped by incubation in a solution containing 0.3 M NaCl, 0.03 M trisodium citrate, pH 7, for 20 min, then embryos were transferred onto glass slides, set in 0.8% low melting agarose, laterally oriented, and analyzed using a NIKON H600L confocal microscope equipped with Laser 488 (excitation 488 nm and emission 520 nm) to visualize GFP and a 40× objective. .. Apoptotic nuclei in entire embryos (head, trunk, yolk and tail) were counted using VOLOCITY 6.0 software (PerkinElmer, Waltham, MA, USA).

    Article Title: The Unique Cysteine of F-ATP Synthase OSCP Subunit Participates in Modulation of the Permeability Transition Pore.
    Article Snippet: After washings in PBS, embryos were incubated with primary antibodies diluted 1:100 in PBS/Tween (polyclonal rabbit anti-TOM20, Santa Cruz Biotechnology, and monoclonal mouse anti-OSCP (A-8), Santa Cruz Biotechnology) at 4 C for 72 h. After extensive washings in PBS, embryos were incubated for 48 h at 4 C with secondary antibodies (anti-mouse TRITC conjugated and anti-rabbit FITC conjugated, Dako) diluted 1:500 in PBS/Tween. .. Full Z stack images were collected with a NIKON H600L confocal microscope (20x objective) equipped with Laser 488 (excitation 488 nm and emission 520 nm) to visualize FITC and Laser 561 (excitation 561 nm and emission 620 nm) for TRITC. .. TdT-mediated dUTP Nick-End Labeling (TUNEL) assay To assess the effects of wild-type andOSCPC138S on apoptosis, TUNEL assay was performed on embryos at 72 hpf using a TUNEL kit protocol (Promega).

    Article Title: The Unique Cysteine of F-ATP Synthase OSCP Subunit Participates in Modulation of the Permeability Transition Pore.
    Article Snippet: .. Then, embryoswere transferred onto glass slides, set in 0.8% low melting agarose, laterally oriented, and analyzed using a NIKON H600L confocal microscope equipped with Laser 488 (excitation 488 nm and emission 520 nm) to visualize GFP and a 40x objective. .. Apoptotic nuclei in the tail area were analyzed and counted using ImageJ software. e6 Cell Reports 32, 108095, September 1, 2020

    Article Title: Ca 2+ binding to F‐ATP synthase β subunit triggers the mitochondrial permeability transition
    Article Snippet: Fish were incubated with primary antibodies diluted 1:100 in PBS/Tween (polyclonal rabbit anti‐TOM20, Santa Cruz Biotechnology, and monoclonal mouse anti‐β ATP synthase subunit, Abcam) at 4°C for 72 h. After extensive washings, embryos were incubated for 48 h at 4°C with secondary antibodies (anti‐mouse TRITC conjugated and anti‐rabbit FITC conjugated, Dako, Cernusco sul Naviglio, Italy) diluted 1:500 in PBS/Tween. .. Full Z‐stack images were collected with a NIKON H600L confocal microscope (40× objective) equipped with Laser 488 (excitation 488 nm and emission 520 nm) to visualize FITC and Laser 561 (excitation 561 nm and emission 620 nm) for TRITC. .. To assess apoptosis, TUNEL staining was performed on embryos at 48 hpf using a TUNEL kit protocol (Promega).



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